Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity

Mutation Detection by Single Strand Conformation Polymorphism and Heteroduplex Analysis

Single strand conformation polymorphism (SSCP) and heteroduplex analysis (HDA) are two of the most popular electrophoresis-based mutation detection methods. Coupled to DNA amplification of the sequence to be analyzed, these techniques have become the methods of choice for a number of molecular diagnostic laboratories. This can be explained mainly by the numerous advantages, namely their technic...

Carrier Determination in a Hemophilia B Family Using Single Strand Conformation Polymorphism (SSCP) and Sequencing

Prediction of DNA single-strand conformation polymorphism: analysis by capillary electrophoresis and computerized DNA modeling.

We have analyzed previously three representative p53 single-point mutations by capillary-electrophoresis single-strand conformation polymorphism (CE-SSCP). In the current study, we compared our CE-SSCP results with the potential secondary structures predicted by an RNA/DNA-folding algorithm with DNA energy rules, used in conjunction with a computer analysis workbench called STRUCTURELAB. Each o...

Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): comparison with DNA-SSCP.

Single-strand conformation polymorphism (SSCP) is a simple method for detecting the presence of mutations in a segment of DNA, but the fraction of all mutations detected is unclear. We have evaluated SSCP for the detection of single-base mutations in the factor IX gene. Multiple conditions were examined including electrophoresis temperature, electrophoresis buffer concentration, acrylamide to b...

Single-strand Conformation Polymorphism (sscp) Analysis: a Rapid and Sensitive Method for Detection of Genetic Diversity among Virus Population

Here we present a review of the single-strand conformation polymorphism (SSCP) method, which allows DNA fragments that have been amplified with specific primers and polymerase chain reaction (PCR) to be scanned rapidly for any sequence variation. The general principles of the method are described, as well as the major factors that must be considered in developing an optimal SSCP strategy, namel...